New discoveries in chemical research and development in 2021.Electric Literature of 546-89-4, The reactant in an enzyme-catalyzed reaction is called a substrate. Enzyme inhibitors cause a decrease in the reaction rate of an enzyme-catalyzed reaction. 546-89-4, Name is Lithiumacetate, molecular formula is C2H3LiO2. In an article, author is Zheng, L,once mentioned of 546-89-4.
Antibody 16E7 catalyzes the carbon protonation of enol ether 2 to hemiacetal 3, and the carbon deprotonation of benzisoxazole 7 to phenol 8. This antibody shows an extreme case of hysteresis, requiring several hours to reach full activity. Antibody 16E7 was expressed as recombinant chimeric Fab in Escherichia coli. A model for the three-dimensional structure was produced by homology modeling and used for a docking procedure to obtain models for antibody-ligand complexes. Site-direct mutagenesis of Glu(L39), identified as a possible catalytic residue by the model, to either glutamine or alanine abolished catalysis, showing that both the protonation reaction of enol ether 2 and the deprotonation of benzisoxazole 7 are promoted by the same residue. The model furthermore suggested that substrate access to the catalytic site might be hindered by a flexible HCDR3 loop held in closed position by a hydrogen bond between Ser(H99) and Glu(L39), which could explain the observed hysteresis effect. In agreement with this model, mutagenesis of Ser(H99) to alanine, or deletion of this residue, was found to reduce hysteresis by approximately 50%. (C) 2004 Elsevier Ltd. All rights reserved.
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Reference:
Benzisoxazole – Wikipedia,
,Benzisoxazole – an overview | ScienceDirect Topics